Reversed Phase Ion-Pair Chromatographic Separation of Acetyl Choline and Choline

A reversed phase High-Performance Liquid Chromatographic procedure for the separation of choline and acetyl choline is described. The method employs a mobile phase containing aromatic sulphonates as counter ions for ion-pair chromatography and for U.V. Visualisation of choline and acetyl choline at...

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Veröffentlicht in:	Journal of liquid chromatography 1985-03, Vol.8 (3), p.537-544
1. Verfasser:	Raghuveeran, C. D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences Fundamental and applied biological sciences. Psychology Non peptidic neurotransmitters, polyamines Other biological molecules
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Beschreibung
Zusammenfassung:	A reversed phase High-Performance Liquid Chromatographic procedure for the separation of choline and acetyl choline is described. The method employs a mobile phase containing aromatic sulphonates as counter ions for ion-pair chromatography and for U.V. Visualisation of choline and acetyl choline at 254 nm. The method enables the separation of the two compounds in the 1-10 ug concentration range in six minutes. The separations are better achieved on a PRP-I resin column.
ISSN:	0148-3919 2331-0413
DOI:	10.1080/01483918508067099