Determination of niacin in cereal samples by HPLC [high-pressure-liquid chromatography]
A liquid chromatographic method for the analysis of niacin in cereal products is presented. The sample is extracted in the presence of Ca(OH) 2 , concentrated and purified on an anion exchange resin, and further cleaned up by oxidation with KMnO 4 . Final quantitation is by HPLC with ultraviolet det...
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| Veröffentlicht in: | Journal of liquid chromatography 1980, Vol.3 (2), p.269-277 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | A liquid chromatographic method for the analysis of niacin in cereal products is presented. The sample is extracted in the presence of Ca(OH)
2
, concentrated and purified on an anion exchange resin, and further cleaned up by oxidation with KMnO
4
. Final quantitation is by HPLC with ultraviolet detection at 254 nm.
Using a μBondapak C
18
column, a mobile phase consisting of 5% methanol in water containing 0.005 M tetrabutyl ammonium phosphate, and a flow rate of 2.0 ml/min., niacin was found to have a retention time of about 7 minutes. |
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| ISSN: | 0148-3919 2331-0413 |
| DOI: | 10.1080/01483918008060170 |