A Rapid Quantitative Assay of Sparsomycin by High Pressure Liquid Chromatography

A Rapid Quantitative Assay of Sparsomycin by High Pressure Liquid Chromatography

Fast quantitative analysis of sparsomycin in fermentation broth can be accurately carried out by Amberlite XAD-2 column chromatography followed by normal phase high pressure liquid chromatography using a Porasil T column (2mm ID × 61 cm) and a mobile phase consisting of ethyl acetate-methanol-concen...

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Veröffentlicht in:Journal of liquid chromatography 1979-01, Vol.2 (1), p.85-90
Hauptverfasser: Chan, J. A., Stroshane, R. M., Guenther, E. C.
Format: Artikel
Sprache:eng
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Zusammenfassung:Fast quantitative analysis of sparsomycin in fermentation broth can be accurately carried out by Amberlite XAD-2 column chromatography followed by normal phase high pressure liquid chromatography using a Porasil T column (2mm ID × 61 cm) and a mobile phase consisting of ethyl acetate-methanol-concentrated ammonium hydroxide (90:10:0.1).
ISSN:0148-3919
DOI:10.1080/01483917908060048