A new assay for transglutaminase
A new solid-phase micro assay for transglutaminase has been developed. Casein bound to microtitre plates and biotin-labelled casein were used as substrates in a transglutaminase-dependent cross-linking reaction. The resulting immobilized biotin was visualized by addition of avidin-labelled alkaline...
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| Veröffentlicht in: | Scandinavian journal of clinical and laboratory investigation 1991-04, Vol.51 (2), p.119-124 |
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| container_end_page | 124 |
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| container_issue | 2 |
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| container_title | Scandinavian journal of clinical and laboratory investigation |
| container_volume | 51 |
| creator | Seiving, B. Stenberg, P. Nilsson, B. |
| description | A new solid-phase micro assay for transglutaminase has been developed. Casein bound to microtitre plates and biotin-labelled casein were used as substrates in a transglutaminase-dependent cross-linking reaction. The resulting immobilized biotin was visualized by addition of avidin-labelled alkaline phosphatase followed by /?-nitrophenyl phosphate. The colour development was monitored at 405 nm. Tissue transglutaminase was used as test enzyme. The method was also applied to normal and Factor XHI-deficient plasma. |
| doi_str_mv | 10.1080/00365519109091096 |
| format | Article |
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Casein bound to microtitre plates and biotin-labelled casein were used as substrates in a transglutaminase-dependent cross-linking reaction. The resulting immobilized biotin was visualized by addition of avidin-labelled alkaline phosphatase followed by /?-nitrophenyl phosphate. The colour development was monitored at 405 nm. Tissue transglutaminase was used as test enzyme. The method was also applied to normal and Factor XHI-deficient plasma.</description><identifier>ISSN: 0036-5513</identifier><identifier>EISSN: 1502-7686</identifier><identifier>DOI: 10.1080/00365519109091096</identifier><identifier>PMID: 1675021</identifier><identifier>CODEN: SJCLAY</identifier><language>eng</language><publisher>Oslo: Informa Healthcare</publisher><subject>Alkaline Phosphatase ; Analytical, structural and metabolic biochemistry ; Animals ; Avidin ; Biological and medical sciences ; Biotin ; casein ; Caseins - metabolism ; Enzymes and enzyme inhibitors ; Factor XIII ; Factor XIII Deficiency - enzymology ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Spectrophotometry ; Transglutaminases - analysis ; Transglutaminases - metabolism</subject><ispartof>Scandinavian journal of clinical and laboratory investigation, 1991-04, Vol.51 (2), p.119-124</ispartof><rights>1991 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1991</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-bfee85523e54cd96f5d635049fc8613f5c63a1c2e01adac24596bf460dd065a3</citedby><cites>FETCH-LOGICAL-c345t-bfee85523e54cd96f5d635049fc8613f5c63a1c2e01adac24596bf460dd065a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/00365519109091096$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/00365519109091096$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,59645,59751,60434,60540,61219,61254,61400,61435</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4525598$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1675021$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Seiving, B.</creatorcontrib><creatorcontrib>Stenberg, P.</creatorcontrib><creatorcontrib>Nilsson, B.</creatorcontrib><title>A new assay for transglutaminase</title><title>Scandinavian journal of clinical and laboratory investigation</title><addtitle>Scand J Clin Lab Invest</addtitle><description>A new solid-phase micro assay for transglutaminase has been developed. Casein bound to microtitre plates and biotin-labelled casein were used as substrates in a transglutaminase-dependent cross-linking reaction. The resulting immobilized biotin was visualized by addition of avidin-labelled alkaline phosphatase followed by /?-nitrophenyl phosphate. The colour development was monitored at 405 nm. Tissue transglutaminase was used as test enzyme. The method was also applied to normal and Factor XHI-deficient plasma.</description><subject>Alkaline Phosphatase</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Avidin</subject><subject>Biological and medical sciences</subject><subject>Biotin</subject><subject>casein</subject><subject>Caseins - metabolism</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Factor XIII</subject><subject>Factor XIII Deficiency - enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Spectrophotometry</subject><subject>Transglutaminases - analysis</subject><subject>Transglutaminases - metabolism</subject><issn>0036-5513</issn><issn>1502-7686</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1LwzAUhoMoc05_gBdCL8S76knzsRa9GcMvGHiz-3CWJq4jbWfSMvbvzdhURPAmIbzPezh5CLmkcEshhzsAJoWgBYUCdoc8IkMqIEvHMpfHZLjL0wiwU3IWwgrim-V8QAZUjiNGhySZJI3ZJBgCbhPb-qTz2IR313dYVw0Gc05OLLpgLg73iMyfHufTl3T29vw6ncxSzbjo0oU1JhciY0ZwXRbSilIyAbywOpeUWaElQ6ozAxRL1BkXhVxYLqEsQQpkI3KzH7v27UdvQqfqKmjjHDam7YPKQQITvIgg3YPatyF4Y9XaVzX6raKgdlLUHymxc3UY3i9qU_409hZifn3IMWh0NirQVfjGuMiEKPKIPeyxqommaty03pWqw61r_VeH_bfF_a_60qDrlhq9Uau29020-88fPgEi2Ix4</recordid><startdate>199104</startdate><enddate>199104</enddate><creator>Seiving, B.</creator><creator>Stenberg, P.</creator><creator>Nilsson, B.</creator><general>Informa Healthcare</general><general>Taylor & Francis</general><general>Scandinavian University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199104</creationdate><title>A new assay for transglutaminase</title><author>Seiving, B. ; Stenberg, P. ; Nilsson, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-bfee85523e54cd96f5d635049fc8613f5c63a1c2e01adac24596bf460dd065a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Alkaline Phosphatase</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Avidin</topic><topic>Biological and medical sciences</topic><topic>Biotin</topic><topic>casein</topic><topic>Caseins - metabolism</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Factor XIII</topic><topic>Factor XIII Deficiency - enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Spectrophotometry</topic><topic>Transglutaminases - analysis</topic><topic>Transglutaminases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Seiving, B.</creatorcontrib><creatorcontrib>Stenberg, P.</creatorcontrib><creatorcontrib>Nilsson, B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Scandinavian journal of clinical and laboratory investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Seiving, B.</au><au>Stenberg, P.</au><au>Nilsson, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new assay for transglutaminase</atitle><jtitle>Scandinavian journal of clinical and laboratory investigation</jtitle><addtitle>Scand J Clin Lab Invest</addtitle><date>1991-04</date><risdate>1991</risdate><volume>51</volume><issue>2</issue><spage>119</spage><epage>124</epage><pages>119-124</pages><issn>0036-5513</issn><eissn>1502-7686</eissn><coden>SJCLAY</coden><abstract>A new solid-phase micro assay for transglutaminase has been developed. Casein bound to microtitre plates and biotin-labelled casein were used as substrates in a transglutaminase-dependent cross-linking reaction. The resulting immobilized biotin was visualized by addition of avidin-labelled alkaline phosphatase followed by /?-nitrophenyl phosphate. The colour development was monitored at 405 nm. Tissue transglutaminase was used as test enzyme. The method was also applied to normal and Factor XHI-deficient plasma.</abstract><cop>Oslo</cop><pub>Informa Healthcare</pub><pmid>1675021</pmid><doi>10.1080/00365519109091096</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-5513 |
| ispartof | Scandinavian journal of clinical and laboratory investigation, 1991-04, Vol.51 (2), p.119-124 |
| issn | 0036-5513 1502-7686 |
| language | eng |
| recordid | cdi_crossref_primary_10_1080_00365519109091096 |
| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Taylor & Francis Journals Complete |
| subjects | Alkaline Phosphatase Analytical, structural and metabolic biochemistry Animals Avidin Biological and medical sciences Biotin casein Caseins - metabolism Enzymes and enzyme inhibitors Factor XIII Factor XIII Deficiency - enzymology Fundamental and applied biological sciences. Psychology Guinea Pigs Spectrophotometry Transglutaminases - analysis Transglutaminases - metabolism |
| title | A new assay for transglutaminase |
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