A new assay for transglutaminase

A new assay for transglutaminase

A new solid-phase micro assay for transglutaminase has been developed. Casein bound to microtitre plates and biotin-labelled casein were used as substrates in a transglutaminase-dependent cross-linking reaction. The resulting immobilized biotin was visualized by addition of avidin-labelled alkaline...

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Veröffentlicht in:Scandinavian journal of clinical and laboratory investigation 1991-04, Vol.51 (2), p.119-124
Hauptverfasser: Seiving, B., Stenberg, P., Nilsson, B.
Format: Artikel
Sprache:eng
Schlagworte:
Alkaline Phosphatase
Analytical, structural and metabolic biochemistry
Animals
Avidin
Biological and medical sciences
Biotin
casein
Caseins - metabolism
Enzymes and enzyme inhibitors
Factor XIII
Factor XIII Deficiency - enzymology
Fundamental and applied biological sciences. Psychology
Guinea Pigs
Spectrophotometry
Transglutaminases - analysis
Transglutaminases - metabolism
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Beschreibung
Zusammenfassung:A new solid-phase micro assay for transglutaminase has been developed. Casein bound to microtitre plates and biotin-labelled casein were used as substrates in a transglutaminase-dependent cross-linking reaction. The resulting immobilized biotin was visualized by addition of avidin-labelled alkaline phosphatase followed by /?-nitrophenyl phosphate. The colour development was monitored at 405 nm. Tissue transglutaminase was used as test enzyme. The method was also applied to normal and Factor XHI-deficient plasma.
ISSN:0036-5513
1502-7686
DOI:10.1080/00365519109091096