Detection of repetitive telomeric DNA on chromosomes in the cat by primed in situ labelling (PRINS)

Detection of repetitive telomeric DNA on chromosomes in the cat by primed in situ labelling (PRINS)

We describe a rapid method for detection of telomeric DNA on chromosomes in the cat. Oligonucleotides were annealed to complementary sequences in fixed metaphase chromosomes of the cat and extended using Taq-DNA polymerase. Digoxygenin-11-dUTP was incorporated as a label for detection of the newly s...

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Veröffentlicht in:Caryologia 1999-01, Vol.52 (1-2), p.93-95
Hauptverfasser: Szolgyenyi, W., Loschenberger, K., Mayr, B.
Format: Artikel
Sprache:eng
Schlagworte:
cat
fluorescence
hybridization
telomeric DNA
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Zusammenfassung:We describe a rapid method for detection of telomeric DNA on chromosomes in the cat. Oligonucleotides were annealed to complementary sequences in fixed metaphase chromosomes of the cat and extended using Taq-DNA polymerase. Digoxygenin-11-dUTP was incorporated as a label for detection of the newly synthesized product. This method represents a quick and reliable alternative to conventional fluorescence in situ hybridization.
ISSN:0008-7114
2165-5391
DOI:10.1080/00087114.1998.10589159