Effects of Ultraviolet Light on r-7,t-8-Dihydroxy-t-9,10-Epoxy-7,8,9,10-Tetrahydrobenzo[a]Pyrene (BPDE)-DNA Adducts Adsorbed on Filter Paper and the Detection of Tetrols by Synchronous Solid-Matrix Phosphorescence

Exposure of BPDE-DNA adducts adsorbed on two different types of filter paper to ultraviolet radiation for 60 minutes showed that the solid-matrix fluorescence of the adducts changed very little with time for the adducts adsorbed on Whatman 1PS filter paper. This indicated that tetrols which could be formed from the adducts were not readily produced under these conditions. Synchronous scanning solid-matrix phosphorescence was investigated for the detection of tetrols in the presence of BPDE-DNA adducts adsorbed on 1PS filter paper. The results showed that tetrol could be detected at sub-nanogram levels with the adducts adsorbed on the filter paper.