The Application of Atomic Absorption Method as a Flow Detector to Gel Chromatography

The applicability of atomic absorption method as a flow detector to gel chromatography was discussed. The effluent from the outlet of a Sephadex G-15 column was directly introduced into a nebulizer of atomic absorption spectrometer. It was found that the atomic absorption flow detector was sensitive and highly selective so as to permit the quantitative determination of metal ions. Some advantageous aspects of this detector were also discussed.