Cultural Conditions and Strain Improvement for Production of Bactobolin

The cultural conditions for the production of bactobolin, an antitumor antibiotic produced by Pseudomonas yoshitomiensis, were studied using a mutant strain, C-17-20. The following cultural conditions were found to be suitable: 2.0% mannitol, 0.25% dried yeast, 0.5% (NH 4 ) 2 SO 4 , 0.4% KCl, 0.02% K 2 HPO 4 and 0.8% CaCO 3 ; temperature, 25°C. The highest bactobolin production under the conditions was 189 μg/ml, although the wild strain, Y-12278, produced only 4 μg/ml in the basal conditions.