Multimerization of AAGTGA and GAAAGT Generates Sequences that Mediate Virus Inducibility by Mimicking an Interferon Promoter Element

Multimerization of AAGTGA and GAAAGT Generates Sequences that Mediate Virus Inducibility by Mimicking an Interferon Promoter Element

Multimeric AAGTGA and GAAAGT, when inserted before a minimal promoter, mediate virus-inducible transcription. We have determined that the active sequence within these multimers is TGAAAGTGAAAGT, which is structurally similar to GAGAAGTGAAAGT, a positive response element delineated in the β-interfero...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1991-02, Vol.88 (4), p.1369-1373
Hauptverfasser: Naf, Dieter, Hardin, Steven E., Weissmann, Charles
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Base Sequence
Biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gene expression
Genes
Globins - genetics
Interferon Inducers
Interferons
Interferons - genetics
L Cells (Cell Line) - physiology
Mice
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mutagenesis, Insertional
Newcastle disease virus - genetics
Nucleotides
Oligodeoxyribonucleotides - chemical synthesis
Oligodeoxyribonucleotides - pharmacology
Oligonucleotide Probes
Oligonucleotides
Plasmids
Promoter Regions, Genetic
promoters
RNA
Simian virus 40
SV40
TATA Box
Transcription, Genetic - drug effects
Transfection
Viruses
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Beschreibung
Zusammenfassung:Multimeric AAGTGA and GAAAGT, when inserted before a minimal promoter, mediate virus-inducible transcription. We have determined that the active sequence within these multimers is TGAAAGTGAAAGT, which is structurally similar to GAGAAGTGAAAGT, a positive response element delineated in the β-interferon gene promoter. Both sequences behave like protoenhancers and are similar as regards induction by virus or interferon regulatory factor 1 when supported by a simian virus 40 enhancer.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.4.1369