Aggregation of IgE Receptors Induces Degranulation in Rat Basophilic Leukemia Cells Permeabilized with α -toxin from Staphylococcus aureus

Aggregation of IgE Receptors Induces Degranulation in Rat Basophilic Leukemia Cells Permeabilized with α -toxin from Staphylococcus aureus

A method has been developed by which rat basophilic leukemia (RBL) cells can be permeabilized to small molecules while maintaining their ability to degranulate in response to aggregation of IgE receptors. α -Toxin was isolated from culture supernatants of Staphylococcus aureus by precipitation with...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1988-03, Vol.85 (5), p.1624-1628
1. Verfasser: Hohman, Robert J.
Format: Artikel
Sprache:eng
Schlagworte:
Aggregation
Animals
Bacterial Toxins - pharmacology
Basophils - physiology
Calcium - pharmacology
Cell Membrane Permeability
Cell membranes
Chromaffin cells
Exocytosis
Hemolysin Proteins
Hepatocytes
Histamine Release
IgE receptors
Inositol - metabolism
Inositols
Mast cells
PC12 cells
Potassium
Potassium Chloride - pharmacology
Rats
Receptors, Fc - physiology
Receptors, IgE
Serotonin - metabolism
Staphylococcus aureus
Toxins
Tumor Cells, Cultured
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Zusammenfassung:A method has been developed by which rat basophilic leukemia (RBL) cells can be permeabilized to small molecules while maintaining their ability to degranulate in response to aggregation of IgE receptors. α -Toxin was isolated from culture supernatants of Staphylococcus aureus by precipitation with (NH4)2SO4 and chromatography on phenyl-Sepharose. The isolated foxin binds to the plasma membrane of RBL cells and polymerizes to form a transmembrane pore that allows small molecules (Mr
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.85.5.1624