Nuclear Matrix DNA from Chicken Erythrocytes Contains β -globin Gene Sequences

Nuclear Matrix DNA from Chicken Erythrocytes Contains β -globin Gene Sequences

Nuclear matrices containing residual DNA were isolated from chicken erythrocytes after extraction of purified nuclei with buffered 2 M NaCl. After further purification of this residual DNA, it was found to contain high concentrations of β -globin gene sequences as assayed by dot hybridization with32...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1984-01, Vol.81 (2), p.304-307
Hauptverfasser: Hentzen, Peter C., Rho, Joon H., Bekhor, Isaac
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biochemistry
Cell Nucleus - physiology
Cell Nucleus - ultrastructure
Chickens
Chromatin
DNA
DNA - metabolism
Electron microscopy
Erythrocytes
Erythrocytes - physiology
Gene expression
Gene Expression Regulation
Genes
Globins - genetics
Nuclear matrix
Nucleotide sequences
Protein Binding
RNA
Solubility
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Nuclear matrices containing residual DNA were isolated from chicken erythrocytes after extraction of purified nuclei with buffered 2 M NaCl. After further purification of this residual DNA, it was found to contain high concentrations of β -globin gene sequences as assayed by dot hybridization with32P-labeled nick-translated pHB1001. Electron microscopy of a random sample of this residual DNA fraction shows the DNA to be intimately associated with protein at various intervals. A hypothesis for enrichment of active genes in residual DNA from purified chromatin or in nuclear matrix DNA is also discussed.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.81.2.304