In situ Hybridization of RD114-Virus RNA with Human Metaphase Chromosomes

In situ Hybridization of RD114-Virus RNA with Human Metaphase Chromosomes

In situ hybridization was used to detect regions of human metaphase chromosomes exhibiting complementarity with radioactively-labeled RNA isolated from RD114-virus. The region of homology, as identified by autoradiography, was confined to one D-group chromosome.

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Bibliographische Detailangaben
Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1973-01, Vol.70 (1), p.11-14
Hauptverfasser: Price, Peter M., Hirschhorn, Kurt, Gabelman, Norman, Waxman, Samuel
Format: Artikel
Sprache:eng
Schlagworte:
Biological Sciences: Genetics
Cell lines
Centrifugation, Density Gradient
Chromosomes
Chromosomes - analysis
Chromosomes, Human, 13-15
Cultured cells
DNA
DNA, Viral - isolation & purification
DNA, Viral - metabolism
Human genetics
Humans
In situ hybridization
Karyotyping
Lymphocytes
Lymphocytes - analysis
Lymphocytes - cytology
Metaphase
Mitosis
Neoplasms, Experimental - microbiology
Nucleic Acid Hybridization
Rhabdomyosarcoma - microbiology
RNA
RNA Viruses - analysis
RNA Viruses - enzymology
RNA Viruses - isolation & purification
RNA, Viral - isolation & purification
RNA, Viral - metabolism
RNA-Directed DNA Polymerase - metabolism
Tritium
Viruses
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Beschreibung
Zusammenfassung:In situ hybridization was used to detect regions of human metaphase chromosomes exhibiting complementarity with radioactively-labeled RNA isolated from RD114-virus. The region of homology, as identified by autoradiography, was confined to one D-group chromosome.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.70.1.11