Specific Banding Patterns of Human Chromosomes

Specific Banding Patterns of Human Chromosomes

Individual pairs of human chromosomes can be reliably identified by a new method that does not require special optical equipment and that results in permanent preparations. This method, which is based on treatment of the chromosomes in situ with NaOH, followed by incubation in sodium chloride-trisod...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1971-09, Vol.68 (9), p.2073-2077
Hauptverfasser: Drets, Maximo E., Shaw, Margery W.
Format: Artikel
Sprache:eng
Schlagworte:
Biological Sciences: Genetics
Chromosome morphology
Chromosomes
Chromosomes, Human, 1-3
Chromosomes, Human, 13-15
Chromosomes, Human, 16-18
Chromosomes, Human, 19-20
Chromosomes, Human, 21-22 and Y
Chromosomes, Human, 4-5
Chromosomes, Human, 6-12 and X
Citrates
Female
Fibroblasts - cytology
Fluorescence
Heterochromatin
Human chromosomes
Humans
Karyotyping
Lymphocytes - cytology
Male
Methods
Polyploidy
Sex Chromosomes
Sodium
Sodium Hydroxide
Staining and Labeling
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Beschreibung
Zusammenfassung:Individual pairs of human chromosomes can be reliably identified by a new method that does not require special optical equipment and that results in permanent preparations. This method, which is based on treatment of the chromosomes in situ with NaOH, followed by incubation in sodium chloride-trisodium citrate and Giemsa staining, results in highly specific banding patterns in characteristic regions of the chromosome arms. It should prove useful for the detection of small structural changes in chromosomes.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.68.9.2073