Esterolytic Activity of Elastase

Esterolytic Activity of Elastase

The esters of L-alanine were found to be excellent substrates for elastase. The pH-dependence study using two methyl esters of this substrate showed the participation in the mechanism of an ionizing group with an apparent pK of 6.85 and a decrease of the activity at pH's greater than 10 (that c...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1969-10, Vol.64 (2), p.683-685
Hauptverfasser: Geneste, Patrick, Bender, Myron L.
Format: Artikel
Sprache:eng
Schlagworte:
Alanine - metabolism
Animals
Biochemistry
Biological Sciences: Biochemistry
Chemicals
Enzymes
Esters
Esters - metabolism
Hydrogen-Ion Concentration
Hydrolysis
In Vitro Techniques
Pancreatic Elastase - metabolism
Phosphates
Solubility
Swine
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The esters of L-alanine were found to be excellent substrates for elastase. The pH-dependence study using two methyl esters of this substrate showed the participation in the mechanism of an ionizing group with an apparent pK of 6.85 and a decrease of the activity at pH's greater than 10 (that could correspond to an apparent pK of 11.4).
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.64.2.683