Sequence and expression of a stem-abundant caffeic acid O-methyltransferase cDNA from perennial ryegrass (Lolium perenne)
A perennial ryegrass (Lolium perenne) cDNA library was screened with a PCR-amplified comt DNA fragment generated from ryegrass cDNA template using degenerate oligonucleotide primers. A full-length cDNA (LpeComt1) was isolated and shown to encode a caffeic acid O-methyltransferase (COMT) enzyme by an...
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Veröffentlicht in: | Australian journal of plant physiology 1998, Vol.25 (2), p.225-235 |
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Zusammenfassung: | A perennial ryegrass (Lolium perenne) cDNA library was
screened with a PCR-amplified comt DNA fragment
generated from ryegrass cDNA template using degenerate oligonucleotide
primers. A full-length cDNA (LpeComt1) was isolated and
shown to encode a caffeic acid O-methyltransferase
(COMT) enzyme by analysis of its predicted amino acid sequence. This cDNA was
expressed in E.coli and COMT activity was demonstrated
in bacterial extracts. The predicted amino acid sequence of the perennial
ryegrass COMT was highly similar (88%) with that of a COMT isolated
from maize. Southern blot hybridisation analysis using this cDNA indicated
that it is a member of a small gene family comprising at least two genes in
perennial ryegrass. The LpeComt1 gene is strongly
expressed in stem tissue, but not obviously in shoot, leaf blade, leaf sheath,
flower or root tissue. |
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ISSN: | 0310-7841 1445-4408 1446-5655 |
DOI: | 10.1071/PP97127 |