Three-dimensional anti-Brownian electrokinetic trapping of a single nanoparticle in solution

A microfluidic device with four electrodes in a tetrahedral arrangement is used to demonstrate three-dimensional (3D) trapping of an individual 40 nm fluorescent nanoparticle in solution. Astigmatic imaging is used to determine the particle position in 3D for real-time control of the electrode poten...

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Veröffentlicht in:Applied physics letters 2013-07, Vol.103 (4)
Hauptverfasser: King, Jason K., Canfield, Brian K., Davis, Lloyd M.
Format: Artikel
Sprache:eng
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Zusammenfassung:A microfluidic device with four electrodes in a tetrahedral arrangement is used to demonstrate three-dimensional (3D) trapping of an individual 40 nm fluorescent nanoparticle in solution. Astigmatic imaging is used to determine the particle position in 3D for real-time control of the electrode potentials, which regulate the magnitude and direction of the electric field and the resulting electrokinetic motion of the particle so as to counteract Brownian diffusion. Trapping within a radius of 5 μm for extended periods (>1 min) is exhibited for particles with diffusivity 5.2 μm2/s and could be improved by increasing the imaging rate of 30 Hz.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.4816325