Au nanorods can be used for long-term cell imaging?
The photostability of Au nanorods in human hepatocellular carcinoma cells under two-photon excitation was investigated to evaluate their potential in cell imaging. The photoluminescence images of the intracellular Au nanorods under the two-photon excitation of an 800 nm femtosecond laser were photob...
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Veröffentlicht in: | Applied physics letters 2011-05, Vol.98 (21), p.213704-213704-3 |
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Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
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Zusammenfassung: | The photostability of Au nanorods in human hepatocellular carcinoma cells under two-photon excitation was investigated to evaluate their potential in cell imaging. The photoluminescence images of the intracellular Au nanorods under the two-photon excitation of an 800 nm femtosecond laser were photobleached rapidly, which is due to the melting of Au nanorods. The images lost their brightness for more than 50% after 15 micrograph scans for the nanorod incubation concentrations of 10, 20, and 40 pM. The confocal reflectance image can prevent the image photobleaching and thus is suitable for long-term cell imaging with Au nanorods. |
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ISSN: | 0003-6951 1077-3118 |
DOI: | 10.1063/1.3593961 |