Förster transfer outside the weak-excitation limit
The efficiency of resonance energy transfer can be used to determine nanometer-scale separations between dye molecules in a donor-acceptor pair. We argue that the standard method for making this determination in single-pair experiments is valid only when excitation by the applied field is much slowe...
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Veröffentlicht in: | The Journal of chemical physics 2009-09, Vol.131 (10), p.104509-104509-5 |
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Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
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Zusammenfassung: | The efficiency of resonance energy transfer can be used to determine nanometer-scale separations between dye molecules in a donor-acceptor pair. We argue that the standard method for making this determination in single-pair experiments is valid only when excitation by the applied field is much slower than the other photophysical processes in the system. We derive a simple relation between measured transfer efficiency and interdye distance that is valid regardless of excitation rate for a broad class of currently accepted models for dye photophysics. Significant deviations from weak-field results are predicted for typical experimental conditions. |
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ISSN: | 0021-9606 1089-7690 |
DOI: | 10.1063/1.3230974 |