Enhancement of fluorescence intensity from an immunoassay chip using high-aspect-ratio nanopillars fabricated by nanoimprinting
High-aspect-ratio structures (nanopillars) were used to enhance the fluorescence intensity of immunoassay chips. Nanoimprinting with elongation phenomenon was applied to fabricate polystyrene nanopillars. Human alpha fetoprotein was detected by a fluorescence immunoassay protocol. Fluorescence inten...
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| Veröffentlicht in: | Applied physics letters 2008-07, Vol.93 (3), p.033904-033904-3 |
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| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | High-aspect-ratio structures (nanopillars) were used to enhance the fluorescence intensity of immunoassay chips. Nanoimprinting with elongation phenomenon was applied to fabricate polystyrene nanopillars. Human alpha fetoprotein was detected by a fluorescence immunoassay protocol. Fluorescence intensities were evaluated for areas with nanopillars of different surface areas. The area with nanopillars of
95
nm
diameter and
4.1
μ
m
height showed fluorescence intensity 34 times higher than that of flat areas. |
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| ISSN: | 0003-6951 1077-3118 |
| DOI: | 10.1063/1.2960354 |