Buried microfluidic channel for integrated patch-clamping assay

The authors present a microfluidic device towards an integrated patch-clamping assay. The device replaces conventional glass patch pipette with a buried microfluidic channel on silicon substrate. The microchannel fabrication involves reforming doped glass under heat and pressure, a process, in princ...

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Veröffentlicht in:Applied physics letters 2006-08, Vol.89 (9), p.093902-093902-3
Hauptverfasser: Ong, Wee-Liat, Kee, Jack-Sheng, Ajay, Agarwal, Ranganathan, Nagarajan, Tang, Kum-Cheong, Yobas, Levent
Format: Artikel
Sprache:eng
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Zusammenfassung:The authors present a microfluidic device towards an integrated patch-clamping assay. The device replaces conventional glass patch pipette with a buried microfluidic channel on silicon substrate. The microchannel fabrication involves reforming doped glass under heat and pressure, a process, in principle, analogous to the heat-pulling/polishing of glass patch pipettes. Unlike etching substrate, this process leaves a smooth glass surface for seal formation with cell membrane. The microchannel is evolved from a trapped void inside the trench during nonconformal deposition of the doped glass. The results of seal formation with mammalian cells captured at such microchannel opening are presented.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.2336213