Buried microfluidic channel for integrated patch-clamping assay
The authors present a microfluidic device towards an integrated patch-clamping assay. The device replaces conventional glass patch pipette with a buried microfluidic channel on silicon substrate. The microchannel fabrication involves reforming doped glass under heat and pressure, a process, in princ...
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Veröffentlicht in: | Applied physics letters 2006-08, Vol.89 (9), p.093902-093902-3 |
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Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
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Zusammenfassung: | The authors present a microfluidic device towards an integrated patch-clamping assay. The device replaces conventional glass patch pipette with a buried microfluidic channel on silicon substrate. The microchannel fabrication involves reforming doped glass under heat and pressure, a process, in principle, analogous to the heat-pulling/polishing of glass patch pipettes. Unlike etching substrate, this process leaves a smooth glass surface for seal formation with cell membrane. The microchannel is evolved from a trapped void inside the trench during nonconformal deposition of the doped glass. The results of seal formation with mammalian cells captured at such microchannel opening are presented. |
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ISSN: | 0003-6951 1077-3118 |
DOI: | 10.1063/1.2336213 |