novel isoflavonoid from Millettia puguensis

Abstract From the roots of MILLETTIA PUGUENSIS (Leguminosae), a novel isoflavonoid (5), 2′-methoxy-4′,5′-methylenedioxy-7,8-[2-(1-methylethenyl)furo]isoflavone, and four known compounds, i. e., lupeol (1), (-)-maackiain (2), 6,7-dimethoxy-3′,4′-methylenedioxyisoflavone (3) and 7,2′-dimethoxy-4′,5′-methylenedioxyisoflavone (4) were isolated and identified by 1 H-, 13 C-NMR and mass spectroscopy. All compounds were evaluated for their antiprotozoal and cytotoxic activities, but only a moderate antileishmanial activity was observed for compound 3 (IC 50 = 32 μM against LEISHMANIA INFANTUM), and a moderate cytotoxicity for compound 2 (IC 50 = 43 μM on MRC-5 cells).