Two new triterpene saponins from Nylandtia spinosa

In a continuation of our study on saponin constituents of medicinal plants of the Polygalaceae family [1–3], we have examined the saponin fraction of the roots of Nylandtia spinosa (L.) Dum.. Two new triterpene saponins were isolated by successive MPLC over silica gel. Their structures were established mainly by 600MHz 2D NMR techniques (COSY, TOCSY, NOESY, HSQC, HMBC) and mass spectrometry as 3- O-ß -D-glucopyranosyl-presenegenin-28- O-ß -D-galactopyranosyl-(1→4)-[α-L-arabinopyranosyl-(1→3)]- ß -D-xylopyranosyl-(1→4)-[ß-D-apiofuranosyl-(1→3)]- α -L-rhamnopyranosyl-(1→2)- ß -D-fucopyranosyl ester and 3- O-ß -D-glucopyranosyl-presenegenin-28 -O-ß- D-galactopyranosyl-(1→4)-[α-L-arabinopyranosyl-(1→3)]- ß -D-xylopyranosyl-(1→4)-[ α -L-rhamnopyranosyl-(1→2)- ß- D-fucopyranosyl ester. References : 1. Mitaine-Offer, A.-C. et al. (2005), Helv. Chim. Acta 88: 2986–2995. 2. Mitaine-Offer, A.-C. et al. (2003), Helv. Chim. Acta 86: 2404–2413. 3. Mitaine-Offer, A.-C. et al. (2002), J. Nat. Prod. 65: 553–557.