Involvement of interleukin-1β mediated nuclear factor κB signalling pathways to down-regulate prostate-specific antigen and cell proliferation in LNCaP prostate cancer cells

Involvement of NF‐κB (nuclear factor κB) mediated by IL‐1β (interleukin‐1β) on cell proliferation and PSA (prostate‐specific antigen) production of LNCaP prostate cell lines and the possible cross‐talk with Akt (also known as protein kinase B) signalling pathway has been investigated. NF‐κB and Akt were analysed by Western blotting from LNCaP cells treated by IL‐1β before proliferation and PSA production were measured. IL‐1β inhibited proliferation and decreased PSA production. The Akt pathway was not sensitive, whereas NF‐κB phosphorylation occurred as a result of treatment. PSA production and proliferation of LNCaP cells were down‐regulated by NF‐κB mediated by IL‐1β promoting anti‐apoptotic signalling and co‐suppressor factors of PSA expression. IL‐1β through NF‐κB activation provides a rationale for therapeutic approaches in the anticancer treatment of prostate.