Mimicking the extracellular matrix by incorporating functionalized graphene into hybrid hydrogels

The efficient functionalization of graphene with sulfonic groups using a sustainable approach facilitates the interaction of biomolecules with its surface. The inclusion of these graphene sheets inside a photopolymerized acrylamide-based hydrogel provides a 3D scaffold with viscoelastic behaviour closer to that found in natural tissues. Cell-culture experiments and differentiation assays with SH-SY5Y cells showed that these hybrid hydrogels are non-cytotoxic, thus making them potentially useful as scaffold materials mimicking the extracellular environment. The efficient functionalization of graphene with sulfonic groups facilitates the interaction of biomolecules with the hydrogel surface. An enhancement on the viscoelasticity and cell development was observed in the hybrid-sulfonic graphene hydrogels.