Detection and tracking of cytoplasmic G-quadruplexes in live cells

Detection and tracking of cytoplasmic G-quadruplexes in live cells

A TTPP probe was developed to distinguish G-quadruplexes (G4s) from other nucleic acid topologies through longer fluorescence lifetimes and higher quantum yields. In fluorescence lifetime imaging microscopy, TTPP enabled the visualization of cytoplasmic G4s in live cells, and showed the potential to...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2023-11, Vol.59 (89), p.13348-13351
Hauptverfasser: Rao, Lu-Si, Hao, Liang, Liu, Liu-Yi, Zeng, You-Liang, Liang, Bing-Bing, Liu, Wenting, Mao, Zong-Wan
Format: Artikel
Sprache:eng
Schlagworte:
Apoptosis
Fluorescence
Nucleic acids
Topology
Tracking
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Beschreibung
Zusammenfassung:A TTPP probe was developed to distinguish G-quadruplexes (G4s) from other nucleic acid topologies through longer fluorescence lifetimes and higher quantum yields. In fluorescence lifetime imaging microscopy, TTPP enabled the visualization of cytoplasmic G4s in live cells, and showed the potential to detect cell apoptosis and ferroptosis by tracking cytoplasmic G4s. By using FLIM, TTPP achieved visualization of cytoplasmic G4s in live cells, and detected cell death by tracking cytoplasmic G4s.
ISSN:1359-7345
1364-548X
DOI:10.1039/d3cc03043a