First electrochemical bioplatforms to determine anti-centromere B antibodies: critical comparison between integrated and magnetic bead-assisted strategies using His-tag chemistry

This work reports and compares the first electrochemical bioplatforms for determining anti-centromere B antibodies developed in magnetic microbead-assisted or integrated formats and proves the competitiveness of the nitrilotriacetic acid chemistry they exploit against common carbodiimide/succinimide-based one and their potential to determine serum target autoantibodies at clinically relevant levels. Development and comparison of the first electrochemical bioplatforms for determining anti-centromere B antibodies (CENPB-Abs) developed in magnetic microbead-assisted or integrated formats using His-tag chemistry.