A two-photon fluorescence probe for cell membrane imaging under temporal-focusing multiphoton excitation microscopy (TFMPEM)

A small-sized chromophore, BTTA-2OH , manifesting favorable solubility, large two-photon excitation efficiency, and good fluorescence photostability was synthesized to label the membrane of living cells for visualizing the dynamic movement of membrane-related vesicles via a two-photon fluorescence imaging technique based on wavelength-tunable temporal-focusing multiphoton excitation microscopy. A small-sized chromophore with comparable photostability and superior two-photon excitation/fluorescence efficiencies was designed and demonstrated to reveal cell membrane-related vesicles via temporal-focusing multiphoton excitation microscopy.