A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer

A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer

A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantifi...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:RSC advances 2020-02, Vol.1 (14), p.887-892
Hauptverfasser: Lou, Ut Kei, Wong, Chi Hin, Chen, Yangchao
Format: Artikel
Sprache:eng
Schlagworte:
Biomarkers
Cancer
Chemistry
Colorimetry
Diagnosis
Nanoparticles
Pancreatic cancer
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantification of lncRNA down to 50 copies. A colorimetric LAMP-(+)AuNP assay was proposed for detection of pancreatic cancer.
ISSN:2046-2069
2046-2069
DOI:10.1039/c9ra07858d