Dual amplification ratiometric biosensor based on a DNA tetrahedron nanostructure and hybridization chain reaction for the ultrasensitive detection of microRNA-133a

Dual amplification ratiometric biosensor based on a DNA tetrahedron nanostructure and hybridization chain reaction for the ultrasensitive detection of microRNA-133a

A novel ratiometric electrochemiluminescence (ECL)-electrochemical (EC) hybrid biosensor with a high accuracy and reproducibility was fabricated for the ultrasensitive detection of miRNA-133a. With the help of a DNA tetrahedron nanostructure and hybridization chain reaction dual amplification strate...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2019-09, Vol.55 (77), p.11551-11554
Hauptverfasser: Zhu, Liping, Ye, Jing, Wang, Shuang, Yan, Mengxia, Zhu, Qiuju, Huang, Jianshe, Yang, Xiurong
Format: Artikel
Sprache:eng
Schlagworte:
Amplification
Biosensors
Electrochemiluminescence
MicroRNAs
Nanostructure
Optimization
Ribonucleic acid
RNA
Tetrahedra
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Beschreibung
Zusammenfassung:A novel ratiometric electrochemiluminescence (ECL)-electrochemical (EC) hybrid biosensor with a high accuracy and reproducibility was fabricated for the ultrasensitive detection of miRNA-133a. With the help of a DNA tetrahedron nanostructure and hybridization chain reaction dual amplification strategy, the detection limit of 12.17 aM for miRNA-133a was obtained. A novel ratiometric electrochemiluminescence-electrochemical hybrid biosensor with high accuracy and reproducibility was fabricated for the ultrasensitive detection of miRNA-133a.
ISSN:1359-7345
1364-548X
DOI:10.1039/c9cc05592d