XNA ligation using T4 DNA ligase in crowding conditions

T4 DNA ligase is capable of ligating 2′OMe-RNA duplexes, HNA, LNA and FANA mixed sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of oligonucleotides containing multiple consecutive XNA nucleotides at the ligation site has not been reported before. T4 DNA ligase is capable of ligating XNA in the presence of 10% w/v PEG8000 and 3 M betaine.