Biomimetically synthesized ZnO nanoparticles attain potent antibacterial activity against less susceptible S. aureus skin infection in experimental animals

In the present study, we explored the antibacterial potential of ZnO-nanoparticles that were biomimetically synthesized employing conditioned culture supernatant of Staphylococcus aureus ( S. aureus ). The in situ synthesized ZnO-NPs were characterized employing various biophysical techniques including UV-vis spectroscopy, electron microscopy, DLS and XRD analysis etc. Electron microscopic studies revealed the disintegration of bacterial cell wall upon interaction with synthesized ZnO-NPs. Various employed tests provided evidence that synthesized ZnO-NPs effectively inhibited less susceptible Gram-positive and Gram-negative bacterial isolates. Moreover, the synthesized ZnO-NPs inhibited the biofilm synthesis of tested microbes in vitro . Finally, the biomimetically synthesized ZnO-NPs successfully treated the S. aureus mediated experimental skin infection in BALB/c mice as well. Biomimetic synthesis of ZnO-NPs and their topical application on S. aureus induced skin infection leads to reduction in infection in mice model.