Multicolor GLUT5-permeable fluorescent probes for fructose transport analysis

The specificity of carbohydrate transporters towards their substrates poses a significant challenge for the development of molecular probes to monitor sugar uptake in cells for biochemical and biomedical applications. Herein we report a new set of coumarin-based fluorescent sugar conjugates applicable for the analysis of fructose uptake due to their free passage through the fructose-specific transporter GLUT5. The reported probes cover a broad range of the fluorescence spectrum providing essential tools for the evaluation of fructose transport capacity in live cells. A set of coumarin-based fluorescent sugar conjugates - ManCous is reported. ManCous are specific for fructose transporter GLUT5 and cover a broad range of the fluorescence spectrum providing essential tools for the evaluation of fructose transport capacity in live cells.