Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe
BODIPY derivative, SF-1 , exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically det...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2018, Vol.54 (15), p.1849-1852 |
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| container_title | Chemical communications (Cambridge, England) |
| container_volume | 54 |
| creator | Ali, Firoj Aute, Sunil Sreedharan, Sreejesh Anila, H. A Saeed, Hiwa K Smythe, Carl G Thomas, Jim A Das, Amitava |
| description | BODIPY derivative,
SF-1
, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles.
SF-1
is compatible with 3D-SIM imaging of individual cells.
A probe that specifically images HOCl in Golgi apparatus and lysosomes that is compatible wih the super resolution microscopy technique, SIM, is reported. |
| doi_str_mv | 10.1039/c7cc09433g |
| format | Article |
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SF-1
, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles.
SF-1
is compatible with 3D-SIM imaging of individual cells.
A probe that specifically images HOCl in Golgi apparatus and lysosomes that is compatible wih the super resolution microscopy technique, SIM, is reported.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/c7cc09433g</identifier><identifier>PMID: 29384535</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Animals ; Boron Compounds - chemistry ; Fluorescence ; Fluorescent Dyes - chemistry ; Hypochlorous Acid - analysis ; Imaging, Three-Dimensional ; Lysosomes ; Macrophages ; Macrophages - chemistry ; Mice ; Microscopy ; Microscopy - methods ; Organelles ; Organelles - chemistry ; RAW 264.7 Cells ; Time Factors</subject><ispartof>Chemical communications (Cambridge, England), 2018, Vol.54 (15), p.1849-1852</ispartof><rights>Copyright Royal Society of Chemistry 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-feccf5db9d2204ebad8300f5217b2d636a1605ba08ab78fe9d19ffc17243d2b83</citedby><cites>FETCH-LOGICAL-c439t-feccf5db9d2204ebad8300f5217b2d636a1605ba08ab78fe9d19ffc17243d2b83</cites><orcidid>0000-0003-3666-1743 ; 0000-0002-8662-7917</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29384535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ali, Firoj</creatorcontrib><creatorcontrib>Aute, Sunil</creatorcontrib><creatorcontrib>Sreedharan, Sreejesh</creatorcontrib><creatorcontrib>Anila, H. A</creatorcontrib><creatorcontrib>Saeed, Hiwa K</creatorcontrib><creatorcontrib>Smythe, Carl G</creatorcontrib><creatorcontrib>Thomas, Jim A</creatorcontrib><creatorcontrib>Das, Amitava</creatorcontrib><title>Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>BODIPY derivative,
SF-1
, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles.
SF-1
is compatible with 3D-SIM imaging of individual cells.
A probe that specifically images HOCl in Golgi apparatus and lysosomes that is compatible wih the super resolution microscopy technique, SIM, is reported.</description><subject>Animals</subject><subject>Boron Compounds - chemistry</subject><subject>Fluorescence</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Hypochlorous Acid - analysis</subject><subject>Imaging, Three-Dimensional</subject><subject>Lysosomes</subject><subject>Macrophages</subject><subject>Macrophages - chemistry</subject><subject>Mice</subject><subject>Microscopy</subject><subject>Microscopy - methods</subject><subject>Organelles</subject><subject>Organelles - chemistry</subject><subject>RAW 264.7 Cells</subject><subject>Time Factors</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1LwzAYh4MoTqcX70rAm1DNZ9scpegmDHaZ4K0kaTI622Ym7WH_vek2Zy5v8ubheckvANxh9IwRFS860xoJRun6DFxhmrKEs_zrfNxzkWSU8Qm4DmGD4sI8vwQTImjOOOVXoFt5qb_rbg3ny6KB2nXadL2Xfe26AKX2LgSoTdMMjfTQ-bXs4sEEWHfQG9nAvm4NHMJokDAMW-NjP7hmGA2wrUeDdtsd3HqnzA24sLIJ5vZYp-Dz_W1VzJPFcvZRvC4SzajoE2u0trxSoiIEMaNklVOELCc4U6RKaSpxiriSKJcqy60RFRbWapwRRiuicjoFjwdvnPozmNCXGzf4Lo4sCcIop4wxFKmnA7V_pje23Pq6lX5XYlSO0ZZFVhT7aGcRfjgqB9Wa6oT-ZRmB-wPggz7d_v8N_QXKPX_4</recordid><startdate>2018</startdate><enddate>2018</enddate><creator>Ali, Firoj</creator><creator>Aute, Sunil</creator><creator>Sreedharan, Sreejesh</creator><creator>Anila, H. A</creator><creator>Saeed, Hiwa K</creator><creator>Smythe, Carl G</creator><creator>Thomas, Jim A</creator><creator>Das, Amitava</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0003-3666-1743</orcidid><orcidid>https://orcid.org/0000-0002-8662-7917</orcidid></search><sort><creationdate>2018</creationdate><title>Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe</title><author>Ali, Firoj ; Aute, Sunil ; Sreedharan, Sreejesh ; Anila, H. A ; Saeed, Hiwa K ; Smythe, Carl G ; Thomas, Jim A ; Das, Amitava</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-feccf5db9d2204ebad8300f5217b2d636a1605ba08ab78fe9d19ffc17243d2b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Boron Compounds - chemistry</topic><topic>Fluorescence</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Hypochlorous Acid - analysis</topic><topic>Imaging, Three-Dimensional</topic><topic>Lysosomes</topic><topic>Macrophages</topic><topic>Macrophages - chemistry</topic><topic>Mice</topic><topic>Microscopy</topic><topic>Microscopy - methods</topic><topic>Organelles</topic><topic>Organelles - chemistry</topic><topic>RAW 264.7 Cells</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ali, Firoj</creatorcontrib><creatorcontrib>Aute, Sunil</creatorcontrib><creatorcontrib>Sreedharan, Sreejesh</creatorcontrib><creatorcontrib>Anila, H. A</creatorcontrib><creatorcontrib>Saeed, Hiwa K</creatorcontrib><creatorcontrib>Smythe, Carl G</creatorcontrib><creatorcontrib>Thomas, Jim A</creatorcontrib><creatorcontrib>Das, Amitava</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ali, Firoj</au><au>Aute, Sunil</au><au>Sreedharan, Sreejesh</au><au>Anila, H. A</au><au>Saeed, Hiwa K</au><au>Smythe, Carl G</au><au>Thomas, Jim A</au><au>Das, Amitava</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2018</date><risdate>2018</risdate><volume>54</volume><issue>15</issue><spage>1849</spage><epage>1852</epage><pages>1849-1852</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>BODIPY derivative,
SF-1
, exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles.
SF-1
is compatible with 3D-SIM imaging of individual cells.
A probe that specifically images HOCl in Golgi apparatus and lysosomes that is compatible wih the super resolution microscopy technique, SIM, is reported.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>29384535</pmid><doi>10.1039/c7cc09433g</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-3666-1743</orcidid><orcidid>https://orcid.org/0000-0002-8662-7917</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1359-7345 |
| ispartof | Chemical communications (Cambridge, England), 2018, Vol.54 (15), p.1849-1852 |
| issn | 1359-7345 1364-548X |
| language | eng |
| recordid | cdi_crossref_primary_10_1039_C7CC09433G |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Boron Compounds - chemistry Fluorescence Fluorescent Dyes - chemistry Hypochlorous Acid - analysis Imaging, Three-Dimensional Lysosomes Macrophages Macrophages - chemistry Mice Microscopy Microscopy - methods Organelles Organelles - chemistry RAW 264.7 Cells Time Factors |
| title | Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe |
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