Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe

Tracking HOCl concentrations across cellular organelles in real time using a super resolution microscopy probe

BODIPY derivative, SF-1 , exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically det...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2018, Vol.54 (15), p.1849-1852
Hauptverfasser: Ali, Firoj, Aute, Sunil, Sreedharan, Sreejesh, Anila, H. A, Saeed, Hiwa K, Smythe, Carl G, Thomas, Jim A, Das, Amitava
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Boron Compounds - chemistry
Fluorescence
Fluorescent Dyes - chemistry
Hypochlorous Acid - analysis
Imaging, Three-Dimensional
Lysosomes
Macrophages
Macrophages - chemistry
Mice
Microscopy
Microscopy - methods
Organelles
Organelles - chemistry
RAW 264.7 Cells
Time Factors
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Beschreibung
Zusammenfassung:BODIPY derivative, SF-1 , exclusively shows a fluorescence ON response to HOCl and images endogenously generated HOCl in RAW 264.7 macrophages. Widefield and super resolution structured illumination microscopy images confirm localization in the Golgi complex and lysosomes, and hence specifically detects HOCl generated in these organelles. SF-1 is compatible with 3D-SIM imaging of individual cells. A probe that specifically images HOCl in Golgi apparatus and lysosomes that is compatible wih the super resolution microscopy technique, SIM, is reported.
ISSN:1359-7345
1364-548X
DOI:10.1039/c7cc09433g