The antioxidative effects of three lactobacilli on high-fat diet induced obese mice

In this paper, three Lactobacillus strains ( L. coryniformis subsp. torquens T3, L. paracasei subsp. paracasei M5 and L. paracasei subsp. paracasei X12) isolated in our laboratory were investigated for antioxidant activity in vitro and in vivo . Hydroxyl and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activities and reducing power were examined in vitro . Three Lactobacillus strains were administered to high-fat diet induced obese mice. The results indicated that T3 and M5 showed the highest hydroxyl radical and DPPH scavenging activities at a dose of 10 9 CFU mL −1 , with inhibition rates of 40.33, 41.11% and 40.76, 44.31%, respectively. The reducing activities of these two strains were also significantly higher than those of X12. Compared with the high-fat diet group, M5 and T3 have effectively controlled body weight and hepatomegaly and significantly decreased blood triglyceride and total cholesterol levels ( p < 0.05). Besides, M5 and T3 obviously improved the antioxidative indexes including MDA levels, T-SOD, GSH-Px, CAT and T-AOC activities. Overall, M5 and T3 have a strong antioxidant activity in vitro and can improve oxidative damage and control body weight in mice, suggesting that they may be potential anti-obesity and antioxidant probiotics. In this paper, three Lactobacillus strains ( L. coryniformis subsp. torquens T3, L. paracasei subsp. paracasei M5 and L. paracasei subsp. paracasei X12) isolated in our laboratory were investigated for antioxidant activity in vitro and in vivo .