Rotaxane probes for protease detection by 129 Xe hyperCEST NMR

Rotaxane probes for protease detection by 129 Xe hyperCEST NMR

We report a CB6 rotaxane for the Xe hyperCEST NMR detection of matrix metalloprotease 2 (MMP-2) activity. MMP-2 is overexpressed in cancer tissue, and hence is a cancer marker. A peptide containing an MMP-2 recognition sequence was incorporated into the rotaxane, synthesized via CB6-promoted click c...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2017-01, Vol.53 (6), p.1076-1079
Hauptverfasser: Slack, Clancy C, Finbloom, Joel A, Jeong, Keunhong, Bruns, Carson J, Wemmer, David E, Pines, Alexander, Francis, Matthew B
Format: Artikel
Sprache:eng
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Zusammenfassung:We report a CB6 rotaxane for the Xe hyperCEST NMR detection of matrix metalloprotease 2 (MMP-2) activity. MMP-2 is overexpressed in cancer tissue, and hence is a cancer marker. A peptide containing an MMP-2 recognition sequence was incorporated into the rotaxane, synthesized via CB6-promoted click chemistry. Upon cleavage of the rotaxane by MMP-2, CB6 became accessible for Xe@CB6 interactions, leading to protease-responsive hyperCEST activation.
ISSN:1359-7345
1364-548X
DOI:10.1039/C6CC09302G