H 2 -driven biotransformation of n -octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O 2 -tolerant hydrogenase and a P450 monooxygenase

H 2 -driven biotransformation of n -octane to 1-octanol by a recombinant Pseudomonas putida strain co-synthesizing an O 2 -tolerant hydrogenase and a P450 monooxygenase

An in vivo biotransformation system is presented that affords the hydroxylation of n -octane to 1-octanol on the basis of NADH-dependent CYP153A monooxygenase and NAD + -reducing hydrogenase heterologously synthesized in a bacterial host. The hydrogenase sustains H 2 -driven NADH cofactor regenerati...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-10, Vol.51 (90), p.16173-16175
Hauptverfasser: Lonsdale, Thomas H., Lauterbach, Lars, Honda Malca, Sumire, Nestl, Bettina M., Hauer, Bernhard, Lenz, Oliver
Format: Artikel
Sprache:eng
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Zusammenfassung:An in vivo biotransformation system is presented that affords the hydroxylation of n -octane to 1-octanol on the basis of NADH-dependent CYP153A monooxygenase and NAD + -reducing hydrogenase heterologously synthesized in a bacterial host. The hydrogenase sustains H 2 -driven NADH cofactor regeneration even in the presence of O 2 , the co-substrate of monooxygenase.
ISSN:1359-7345
1364-548X
DOI:10.1039/C5CC06078H