Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection
T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch and utilized for developing a target cyclic amplification biosensor strategy for sensitive SNP detection based on graphene oxide quenching of uncleaved probes. T7 exonuclease is reported for...
Gespeichert in:
Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2015-02, Vol.51 (14), p.2954-2956 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 2956 |
---|---|
container_issue | 14 |
container_start_page | 2954 |
container_title | Chemical communications (Cambridge, England) |
container_volume | 51 |
creator | Wu, Zhen-Kun Zhou, Dian-Ming Wu, Zhan Chu, Xia Yu, Ru-Qin Jiang, Jian-Hui |
description | T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch and utilized for developing a target cyclic amplification biosensor strategy for sensitive SNP detection based on graphene oxide quenching of uncleaved probes.
T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch. |
doi_str_mv | 10.1039/c4cc09984b |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1039_C4CC09984B</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1893898840</sourcerecordid><originalsourceid>FETCH-LOGICAL-c324t-f702807422ee3732ee6c02f6fe338268c78f6f7fb7de73903985cabe0b422f4d3</originalsourceid><addsrcrecordid>eNqFkctLxDAQxoMorq5evCv1JkI1zaNJj1p8wYIHV_BW0-lEI32sTRfd_96s6-OmOUxm-H4zMN8QspfQk4Ty7BQEAM0yLco1spXwVMRS6If1ZS6zWHEhR2Tb-xcaXiL1JhkxKTNBdbJFHu9c-1RjXBqPUeN8YwZ4jirnoXeNa83gujYqF9FURfjetXOocUm-ueE5Gkz_hEMEC6gdRKaZ1c46WLVUOCAssx2yYU3tcffrH5P7y4tpfh1Pbq9u8rNJDJyJIbaKMk2VYAyRKx5iCpTZ1CLnmqUalA6FsqWqUPEsrK0lmBJpGVqsqPiYHK3mzvrudY5-KMI2gHVtWuzmvkh0xnWmtaD_o6lkQiZMpAE9XqHQd973aItZ8MX0iyKhxdL8Ihd5_mn-eYAPvubOywarH_Tb7QDsr4Dew4_6e72gH_6lF7PK8g81P5UU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1652451246</pqid></control><display><type>article</type><title>Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Wu, Zhen-Kun ; Zhou, Dian-Ming ; Wu, Zhan ; Chu, Xia ; Yu, Ru-Qin ; Jiang, Jian-Hui</creator><creatorcontrib>Wu, Zhen-Kun ; Zhou, Dian-Ming ; Wu, Zhan ; Chu, Xia ; Yu, Ru-Qin ; Jiang, Jian-Hui</creatorcontrib><description>T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch and utilized for developing a target cyclic amplification biosensor strategy for sensitive SNP detection based on graphene oxide quenching of uncleaved probes.
T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/c4cc09984b</identifier><identifier>PMID: 25594081</identifier><language>eng</language><publisher>England</publisher><subject>Amplification ; Biosensing Techniques - methods ; Biosensors ; Discrimination ; Electrochemistry ; Exodeoxyribonucleases - metabolism ; Graphene ; Graphite - chemistry ; Kinetics ; Mutation ; Nucleic Acid Amplification Techniques ; Oxides ; Oxides - chemistry ; Polymorphism, Single Nucleotide ; Quenching ; Strategy ; Target detection</subject><ispartof>Chemical communications (Cambridge, England), 2015-02, Vol.51 (14), p.2954-2956</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c324t-f702807422ee3732ee6c02f6fe338268c78f6f7fb7de73903985cabe0b422f4d3</citedby><cites>FETCH-LOGICAL-c324t-f702807422ee3732ee6c02f6fe338268c78f6f7fb7de73903985cabe0b422f4d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25594081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Zhen-Kun</creatorcontrib><creatorcontrib>Zhou, Dian-Ming</creatorcontrib><creatorcontrib>Wu, Zhan</creatorcontrib><creatorcontrib>Chu, Xia</creatorcontrib><creatorcontrib>Yu, Ru-Qin</creatorcontrib><creatorcontrib>Jiang, Jian-Hui</creatorcontrib><title>Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch and utilized for developing a target cyclic amplification biosensor strategy for sensitive SNP detection based on graphene oxide quenching of uncleaved probes.
T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch.</description><subject>Amplification</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensors</subject><subject>Discrimination</subject><subject>Electrochemistry</subject><subject>Exodeoxyribonucleases - metabolism</subject><subject>Graphene</subject><subject>Graphite - chemistry</subject><subject>Kinetics</subject><subject>Mutation</subject><subject>Nucleic Acid Amplification Techniques</subject><subject>Oxides</subject><subject>Oxides - chemistry</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Quenching</subject><subject>Strategy</subject><subject>Target detection</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctLxDAQxoMorq5evCv1JkI1zaNJj1p8wYIHV_BW0-lEI32sTRfd_96s6-OmOUxm-H4zMN8QspfQk4Ty7BQEAM0yLco1spXwVMRS6If1ZS6zWHEhR2Tb-xcaXiL1JhkxKTNBdbJFHu9c-1RjXBqPUeN8YwZ4jirnoXeNa83gujYqF9FURfjetXOocUm-ueE5Gkz_hEMEC6gdRKaZ1c46WLVUOCAssx2yYU3tcffrH5P7y4tpfh1Pbq9u8rNJDJyJIbaKMk2VYAyRKx5iCpTZ1CLnmqUalA6FsqWqUPEsrK0lmBJpGVqsqPiYHK3mzvrudY5-KMI2gHVtWuzmvkh0xnWmtaD_o6lkQiZMpAE9XqHQd973aItZ8MX0iyKhxdL8Ihd5_mn-eYAPvubOywarH_Tb7QDsr4Dew4_6e72gH_6lF7PK8g81P5UU</recordid><startdate>20150218</startdate><enddate>20150218</enddate><creator>Wu, Zhen-Kun</creator><creator>Zhou, Dian-Ming</creator><creator>Wu, Zhan</creator><creator>Chu, Xia</creator><creator>Yu, Ru-Qin</creator><creator>Jiang, Jian-Hui</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20150218</creationdate><title>Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection</title><author>Wu, Zhen-Kun ; Zhou, Dian-Ming ; Wu, Zhan ; Chu, Xia ; Yu, Ru-Qin ; Jiang, Jian-Hui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c324t-f702807422ee3732ee6c02f6fe338268c78f6f7fb7de73903985cabe0b422f4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amplification</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensors</topic><topic>Discrimination</topic><topic>Electrochemistry</topic><topic>Exodeoxyribonucleases - metabolism</topic><topic>Graphene</topic><topic>Graphite - chemistry</topic><topic>Kinetics</topic><topic>Mutation</topic><topic>Nucleic Acid Amplification Techniques</topic><topic>Oxides</topic><topic>Oxides - chemistry</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Quenching</topic><topic>Strategy</topic><topic>Target detection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Zhen-Kun</creatorcontrib><creatorcontrib>Zhou, Dian-Ming</creatorcontrib><creatorcontrib>Wu, Zhan</creatorcontrib><creatorcontrib>Chu, Xia</creatorcontrib><creatorcontrib>Yu, Ru-Qin</creatorcontrib><creatorcontrib>Jiang, Jian-Hui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Zhen-Kun</au><au>Zhou, Dian-Ming</au><au>Wu, Zhan</au><au>Chu, Xia</au><au>Yu, Ru-Qin</au><au>Jiang, Jian-Hui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2015-02-18</date><risdate>2015</risdate><volume>51</volume><issue>14</issue><spage>2954</spage><epage>2956</epage><pages>2954-2956</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch and utilized for developing a target cyclic amplification biosensor strategy for sensitive SNP detection based on graphene oxide quenching of uncleaved probes.
T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch.</abstract><cop>England</cop><pmid>25594081</pmid><doi>10.1039/c4cc09984b</doi><tpages>3</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1359-7345 |
ispartof | Chemical communications (Cambridge, England), 2015-02, Vol.51 (14), p.2954-2956 |
issn | 1359-7345 1364-548X |
language | eng |
recordid | cdi_crossref_primary_10_1039_C4CC09984B |
source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
subjects | Amplification Biosensing Techniques - methods Biosensors Discrimination Electrochemistry Exodeoxyribonucleases - metabolism Graphene Graphite - chemistry Kinetics Mutation Nucleic Acid Amplification Techniques Oxides Oxides - chemistry Polymorphism, Single Nucleotide Quenching Strategy Target detection |
title | Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T22%3A48%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Single-base%20mismatch%20discrimination%20by%20T7%20exonuclease%20with%20target%20cyclic%20amplification%20detection&rft.jtitle=Chemical%20communications%20(Cambridge,%20England)&rft.au=Wu,%20Zhen-Kun&rft.date=2015-02-18&rft.volume=51&rft.issue=14&rft.spage=2954&rft.epage=2956&rft.pages=2954-2956&rft.issn=1359-7345&rft.eissn=1364-548X&rft_id=info:doi/10.1039/c4cc09984b&rft_dat=%3Cproquest_cross%3E1893898840%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1652451246&rft_id=info:pmid/25594081&rfr_iscdi=true |