Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection

T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch and utilized for developing a target cyclic amplification biosensor strategy for sensitive SNP detection based on graphene oxide quenching of uncleaved probes. T7 exonuclease is reported for...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-02, Vol.51 (14), p.2954-2956
Hauptverfasser: Wu, Zhen-Kun, Zhou, Dian-Ming, Wu, Zhan, Chu, Xia, Yu, Ru-Qin, Jiang, Jian-Hui
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container_issue 14
container_start_page 2954
container_title Chemical communications (Cambridge, England)
container_volume 51
creator Wu, Zhen-Kun
Zhou, Dian-Ming
Wu, Zhan
Chu, Xia
Yu, Ru-Qin
Jiang, Jian-Hui
description T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch and utilized for developing a target cyclic amplification biosensor strategy for sensitive SNP detection based on graphene oxide quenching of uncleaved probes. T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch.
doi_str_mv 10.1039/c4cc09984b
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source MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects Amplification
Biosensing Techniques - methods
Biosensors
Discrimination
Electrochemistry
Exodeoxyribonucleases - metabolism
Graphene
Graphite - chemistry
Kinetics
Mutation
Nucleic Acid Amplification Techniques
Oxides
Oxides - chemistry
Polymorphism, Single Nucleotide
Quenching
Strategy
Target detection
title Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection
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