Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection

Single-base mismatch discrimination by T7 exonuclease with target cyclic amplification detection

T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch and utilized for developing a target cyclic amplification biosensor strategy for sensitive SNP detection based on graphene oxide quenching of uncleaved probes. T7 exonuclease is reported for...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-02, Vol.51 (14), p.2954-2956
Hauptverfasser: Wu, Zhen-Kun, Zhou, Dian-Ming, Wu, Zhan, Chu, Xia, Yu, Ru-Qin, Jiang, Jian-Hui
Format: Artikel
Sprache:eng
Schlagworte:
Amplification
Biosensing Techniques - methods
Biosensors
Discrimination
Electrochemistry
Exodeoxyribonucleases - metabolism
Graphene
Graphite - chemistry
Kinetics
Mutation
Nucleic Acid Amplification Techniques
Oxides
Oxides - chemistry
Polymorphism, Single Nucleotide
Quenching
Strategy
Target detection
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Zusammenfassung:T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch and utilized for developing a target cyclic amplification biosensor strategy for sensitive SNP detection based on graphene oxide quenching of uncleaved probes. T7 exonuclease is reported for the first time to have high specificity in discriminating single-base mismatch.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc09984b