Recovery of homogeneous and functional β2-adrenergic receptors from extracellular baculovirus particles

Expression in haculovirus-infected insect cells allows sufficient production of G-protein coupled receptor for structural studies. An important drawback of this expression system comes from the presence of unprocessed and biologically inactive receptors that have to be eliminated during receptor pur...

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Veröffentlicht in:	Nature biotechnology 1997-11, Vol.15 (12), p.1300-1304
Hauptverfasser:	Loisel, Thomas P, Ansanay, Hervé, St-Onge, Stéphane, Gay, Bernard, Boulanger, Pierre, Strosberg, A. Donny, Marullo, Stefano, Bouvier, Michel
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Life Sciences Methods. Procedures. Technologies Modification of gene expression level research-article
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Zusammenfassung:	Expression in haculovirus-infected insect cells allows sufficient production of G-protein coupled receptor for structural studies. An important drawback of this expression system comes from the presence of unprocessed and biologically inactive receptors that have to be eliminated during receptor purification steps. We show that vire particles released from Sf9 cells infected with a recombinant baculovirus coding for the human β2-adrenergic receptor (β2AR) cDNA contain glycosylated and biologically active β2AR. In addition, post-translational modifications known to modulate receptor activity were found to occur in these particles.
ISSN:	1087-0156 1546-1696
DOI:	10.1038/nbt1197-1300