The Use of Non-Radioactive Oligonucleotide Probes to Analyze Enzymatically Amplified DNA for Prenatal Diagnosis and Forensic HLA Typing

The Use of Non-Radioactive Oligonucleotide Probes to Analyze Enzymatically Amplified DNA for Prenatal Diagnosis and Forensic HLA Typing

Non-radioactive oligonucleotide probes for specific alleles of the HLA-DQα and β-globin loci have been synthesized and applied to forensic analysis and prenatal diagnosis. The results obtained with biotin-labeled, enzyme-labeled, and 32 P-labeled probes are compared in the individuality analysis of...

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Veröffentlicht in:Bio/Technology 1988-08, Vol.6 (8), p.943-947
Hauptverfasser: Bugawan, Teodorica L, Saiki, Randall K, Levenson, Corey H, Watson, Robert M, Erlich, Henry A
Format: Artikel
Sprache:eng
Schlagworte:
Agriculture
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Life Sciences
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Zusammenfassung:Non-radioactive oligonucleotide probes for specific alleles of the HLA-DQα and β-globin loci have been synthesized and applied to forensic analysis and prenatal diagnosis. The results obtained with biotin-labeled, enzyme-labeled, and 32 P-labeled probes are compared in the individuality analysis of different autopsy samples and the diagnosis of two fetuses at risk for sickle-cell anemia.
ISSN:0733-222X
1087-0156
2331-3684
1546-1696
DOI:10.1038/nbt0888-943