Crystallization of a lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium
The major lignin peroxidase from carbon limited cultures of the white–rot fungus Phanerochaete chrysosporium was purified by isoelectric focusing and crystallized by the hanging drop method. The nature of the crystalline material as lignin peroxidase was established by solid–state micro–spectrophoto...
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| Veröffentlicht in: | Bio/Technology 1988-05, Vol.6 (5), p.571-573 |
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| creator | Troller, J Smit, J.D.G Leisola, M.S.A Kallen, J Winterhalter, K.H Fiechter, A |
| description | The major lignin peroxidase from carbon limited cultures of the white–rot fungus
Phanerochaete chrysosporium
was purified by isoelectric focusing and crystallized by the hanging drop method. The nature of the crystalline material as lignin peroxidase was established by solid–state micro–spectrophotometry and activity measurements on redissolved crystals. The ortho–rhombic, brown crystals had space group P2
1
2
1
2
1
and four lignin peroxidase molecules per unit cell. |
| doi_str_mv | 10.1038/nbt0588-571 |
| format | Article |
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Phanerochaete chrysosporium
was purified by isoelectric focusing and crystallized by the hanging drop method. The nature of the crystalline material as lignin peroxidase was established by solid–state micro–spectrophotometry and activity measurements on redissolved crystals. The ortho–rhombic, brown crystals had space group P2
1
2
1
2
1
and four lignin peroxidase molecules per unit cell.</description><identifier>ISSN: 0733-222X</identifier><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 2331-3684</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt0588-571</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Agriculture ; Agronomy. Soil science and plant productions ; Bioinformatics ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; Chemical, physicochemical, biochemical and biological properties ; ENFERMEDADES FUNGOSAS ; Fundamental and applied biological sciences. Psychology ; Life Sciences ; LIGNINAS ; LIGNINE ; LIGNINS ; Mission oriented research ; MYCOSE ; MYCOSES ; PEROXIDASAS ; PEROXIDASES ; PEROXYDASE ; Physics, chemistry, biochemistry and biology of agricultural and forest soils ; Physiology and metabolism ; research-paper ; Soil science</subject><ispartof>Bio/Technology, 1988-05, Vol.6 (5), p.571-573</ispartof><rights>Nature Publishing Company 1988</rights><rights>1988 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-8b8a898a0ba6cfe27da2f38392b34f4dd4dd3856923377fb75d0ee3575517a53</citedby><cites>FETCH-LOGICAL-c347t-8b8a898a0ba6cfe27da2f38392b34f4dd4dd3856923377fb75d0ee3575517a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nbt0588-571$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nbt0588-571$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7648133$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7673073$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Troller, J</creatorcontrib><creatorcontrib>Smit, J.D.G</creatorcontrib><creatorcontrib>Leisola, M.S.A</creatorcontrib><creatorcontrib>Kallen, J</creatorcontrib><creatorcontrib>Winterhalter, K.H</creatorcontrib><creatorcontrib>Fiechter, A</creatorcontrib><title>Crystallization of a lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium</title><title>Bio/Technology</title><addtitle>Nat Biotechnol</addtitle><description>The major lignin peroxidase from carbon limited cultures of the white–rot fungus
Phanerochaete chrysosporium
was purified by isoelectric focusing and crystallized by the hanging drop method. The nature of the crystalline material as lignin peroxidase was established by solid–state micro–spectrophotometry and activity measurements on redissolved crystals. The ortho–rhombic, brown crystals had space group P2
1
2
1
2
1
and four lignin peroxidase molecules per unit cell.</description><subject>Agriculture</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>Chemical, physicochemical, biochemical and biological properties</subject><subject>ENFERMEDADES FUNGOSAS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Life Sciences</subject><subject>LIGNINAS</subject><subject>LIGNINE</subject><subject>LIGNINS</subject><subject>Mission oriented research</subject><subject>MYCOSE</subject><subject>MYCOSES</subject><subject>PEROXIDASAS</subject><subject>PEROXIDASES</subject><subject>PEROXYDASE</subject><subject>Physics, chemistry, biochemistry and biology of agricultural and forest soils</subject><subject>Physiology and metabolism</subject><subject>research-paper</subject><subject>Soil science</subject><issn>0733-222X</issn><issn>1087-0156</issn><issn>2331-3684</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LxDAURYMoOI6u3LnKwp1W06Rt0qUMfsGAgiO4EMprm7QZOklJWnT89WaouBMh8BY57z7uQeg0JlcxYeLalANJhYhSHu-hGWUsjlgmkn00I5yxiFL6doiOvF8TkvCMJjP0vnBbP0DX6S8YtDXYKgy4043RBvfS2U9dg5dYObvBQyvxR6sHGTk7YDWaZvT4uQUTuKoFOUhctSHP-t46PW6O0YGCzsuTnzlHq7vb1eIhWj7dPy5ullHFEj5EohQgcgGkhKxSkvIaqGKC5bRkiUrqOjwm0iwPhThXJU9rIiVLeZrGHFI2RxdTbOWs906qond6A25bxKTYeSl-vBTBS6DPJ7oHX0GnHJhK-98VnnG2k_UvloiY7bDLCfPhxzTSFWs7OhPa_nH8bMIV2AIaFxJfX0SomtCcfQMQBYhK</recordid><startdate>19880501</startdate><enddate>19880501</enddate><creator>Troller, J</creator><creator>Smit, J.D.G</creator><creator>Leisola, M.S.A</creator><creator>Kallen, J</creator><creator>Winterhalter, K.H</creator><creator>Fiechter, A</creator><general>Nature Publishing Group US</general><general>Nature Publications</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19880501</creationdate><title>Crystallization of a lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium</title><author>Troller, J ; Smit, J.D.G ; Leisola, M.S.A ; Kallen, J ; Winterhalter, K.H ; Fiechter, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-8b8a898a0ba6cfe27da2f38392b34f4dd4dd3856923377fb75d0ee3575517a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Agriculture</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>Chemical, physicochemical, biochemical and biological properties</topic><topic>ENFERMEDADES FUNGOSAS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Life Sciences</topic><topic>LIGNINAS</topic><topic>LIGNINE</topic><topic>LIGNINS</topic><topic>Mission oriented research</topic><topic>MYCOSE</topic><topic>MYCOSES</topic><topic>PEROXIDASAS</topic><topic>PEROXIDASES</topic><topic>PEROXYDASE</topic><topic>Physics, chemistry, biochemistry and biology of agricultural and forest soils</topic><topic>Physiology and metabolism</topic><topic>research-paper</topic><topic>Soil science</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Troller, J</creatorcontrib><creatorcontrib>Smit, J.D.G</creatorcontrib><creatorcontrib>Leisola, M.S.A</creatorcontrib><creatorcontrib>Kallen, J</creatorcontrib><creatorcontrib>Winterhalter, K.H</creatorcontrib><creatorcontrib>Fiechter, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Bio/Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Troller, J</au><au>Smit, J.D.G</au><au>Leisola, M.S.A</au><au>Kallen, J</au><au>Winterhalter, K.H</au><au>Fiechter, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crystallization of a lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium</atitle><jtitle>Bio/Technology</jtitle><stitle>Nat Biotechnol</stitle><date>1988-05-01</date><risdate>1988</risdate><volume>6</volume><issue>5</issue><spage>571</spage><epage>573</epage><pages>571-573</pages><issn>0733-222X</issn><issn>1087-0156</issn><eissn>2331-3684</eissn><eissn>1546-1696</eissn><abstract>The major lignin peroxidase from carbon limited cultures of the white–rot fungus
Phanerochaete chrysosporium
was purified by isoelectric focusing and crystallized by the hanging drop method. The nature of the crystalline material as lignin peroxidase was established by solid–state micro–spectrophotometry and activity measurements on redissolved crystals. The ortho–rhombic, brown crystals had space group P2
1
2
1
2
1
and four lignin peroxidase molecules per unit cell.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><doi>10.1038/nbt0588-571</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0733-222X |
| ispartof | Bio/Technology, 1988-05, Vol.6 (5), p.571-573 |
| issn | 0733-222X 1087-0156 2331-3684 1546-1696 |
| language | eng |
| recordid | cdi_crossref_primary_10_1038_nbt0588_571 |
| source | Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | Agriculture Agronomy. Soil science and plant productions Bioinformatics Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Chemical, physicochemical, biochemical and biological properties ENFERMEDADES FUNGOSAS Fundamental and applied biological sciences. Psychology Life Sciences LIGNINAS LIGNINE LIGNINS Mission oriented research MYCOSE MYCOSES PEROXIDASAS PEROXIDASES PEROXYDASE Physics, chemistry, biochemistry and biology of agricultural and forest soils Physiology and metabolism research-paper Soil science |
| title | Crystallization of a lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium |
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