Crystallization of a lignin peroxidase from the white-rot fungus Phanerochaete chrysosporium

The major lignin peroxidase from carbon limited cultures of the white–rot fungus Phanerochaete chrysosporium was purified by isoelectric focusing and crystallized by the hanging drop method. The nature of the crystalline material as lignin peroxidase was established by solid–state micro–spectrophotometry and activity measurements on redissolved crystals. The ortho–rhombic, brown crystals had space group P2 1 2 1 2 1 and four lignin peroxidase molecules per unit cell.