Dendritic cells facilitate accumulation of IL-17T cells in the kidney following acute renal obstruction
Acute urinary obstruction causes interstitial inflammation with leukocyte accumulation and the secretion of soluble mediators. Here we show that unilateral ureteral ligation caused a progressive increase in renal F4/80+ and F4/80− dendritic cells, monocytes, neutrophils and T-cells 24–72h following...
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Veröffentlicht in: | Kidney international 2008-11, Vol.74 (10), p.1294-1309 |
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Sprache: | eng |
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Zusammenfassung: | Acute urinary obstruction causes interstitial inflammation with leukocyte accumulation and the secretion of soluble mediators. Here we show that unilateral ureteral ligation caused a progressive increase in renal F4/80+ and F4/80− dendritic cells, monocytes, neutrophils and T-cells 24–72h following obstruction. Depletion of dendritic cells by clodronate pretreatment showed these cells to be the most potent source of tumor necrosis factor and other pro-inflammatory mediators in the obstructed kidney. F4/80+ dendritic cells and T-cells co-localized in the cortico-medullary junction and cortex of the obstructed kidney. Cytokine secretion patterns and surface phenotypes of T-cells from obstructed kidneys were found to include interferon-γ-secreting CD4+ and CD8+ memory T-cells as well as interleukin 17 (IL-17)-secreting CD4+ memory T-cells. Depletion of the intra-renal dendritic cells prior to ligation did not numerically reduce T-cells in obstructed kidneys but attenuated interferon-γ and IL-17-competent T-cells. Our study shows that intra-renal dendritic cells are a previously unidentified early source of proinflammatory mediators after acute urinary obstruction and play a specific role in recruitment and activation of effector-memory T-cells including IL-17-secreting CD4+ T-cells. |
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ISSN: | 0085-2538 1523-1755 |
DOI: | 10.1038/ki.2008.394 |