Effect of prostaglandin endoperoxides and metabolites on bone resorption in vitro
PROSTAGLANDINS are potent bone resorbers 1 which have been implicated as mediators of bone loss and hypercalcaemia in disease 2–6 , but the specific products in the pathway of prostaglandin synthesis and degradation which are responsible have not yet been identified in vivo . Although PGE 2 is the m...
Gespeichert in:
Veröffentlicht in: | Nature (London) 1977, Vol.267 (5611), p.532-534 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | PROSTAGLANDINS are potent bone resorbers
1
which have been implicated as mediators of bone loss and hypercalcaemia in disease
2–6
, but the specific products in the pathway of prostaglandin synthesis and degradation which are responsible have not yet been identified
in vivo
. Although PGE
2
is the most potent stimulator of bone resorption among the prostaglandins thus far tested
in vitro
1
, in animal tumour models, the increase in immunoreactive PGE
2
concentrations does not correlate well with the development of hypercalcaemia
2
. We therefore tested the early products of arachidonic acid metabolism via the cyclo-oxygenase pathway, the prostaglandin endoperoxides (PGG
2
and PGH
2
) and some of the 13,14-dihydro (H-PGE
2
and Ha-PGE
2
) and 15-keto-13,14-dihydro metabolites of prostaglandins (15K-H
2
-PGE
2
and 15K-H-PGF
2α
) for their effects on the release of previously incorporated
45
Ca from cultured foetal rat long bones. The endoperoxides were found to cause a rapid transient increase in the release of previously incorporated
45
Ca from bone, but did not stimulate prolonged resorption. H
2
-PGE
2
and H
2
-PGE
1
were almost as potent as the parent compounds. 15K-H
2
-PGE
2
and 15K-H-PGF
2α
were much less potent, but did stimulate resorption at 10
−5
M. |
---|---|
ISSN: | 0028-0836 1476-4687 |
DOI: | 10.1038/267532a0 |