Homotransplant Antigens: Preparation of Active Cellular Fractions

WE have reported recently a method for the measurement of the antigenicity of whole splenic cells using highly inbred strains of mice in a homo-transplant system 1 . Using this method of assay, we have tested fractions of mouse splenic cells for antigenic activity. Below are described the preparation and properties of tissue fractions active as antigens in a homotransplant system. Other methods of preparation of antigenic fractions from tissues have been described by Billingham, Brent and Medawar 2 , by Berrian and Brent 3 , by Hašková and Hrubešová 4 , by Castermans and Oth 5 , and Lejeune-Ledant and Albert 6 .