Bradykinin-releasing Factor from Bothrops jararaca venom

IT was first shown by Rocha e Silva, Beraldo and Rosenfeld 1 that the venom of the snake Bothrops jararaca contains a bradykinin-releasing substance. Holtz and Raudonat 2 verified that the bradykinin releasing substance is precipitated with ammonium sulphate at the level of 60 per cent, is independent of the proteolytic activity on hæmoglobin and accompanies the coagulating activity. Hamberg and Rocha e Silva 3 observed that the bradykinin-releasing factor is heat-resistant and that the decrease of its activity in presence of prolonged heating is parallel to that of the coagulating activity and to the fall of esterase activity on L -benzoylarginine methyl ester. Recently, Contzen, Holtz and Raudonat 4 found that the bradykinin-releasing substance is dialysable and ultra-filtrable, being independent of the coagulating factor but being accompanied by the hydrolysing activity on L -benzoyl-arginine methyl ester.