Gγ13 colocalizes with gustducin in taste receptor cells and mediates IP3 responses to bitter denatonium

Gustducin is a transducin-like G protein selectively expressed in taste receptor cells. The α subunit of gustducin (α-gustducin) is critical for transduction of responses to bitter or sweet compounds. We identified a G-protein γ subunit (Gγ13) that colocalized with α-gustducin in taste receptor cell...

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Veröffentlicht in:Nature neuroscience 1999-12, Vol.2 (12), p.1055-1062
Hauptverfasser: Huang, Liquan, Shanker, Y. Gopi, Dubauskaite, Jolanta, Zheng, Jenny Z., Yan, Wentao, Rosenzweig, Sophia, Spielman, Andrew I., Max, Marianna, Margolskee, Robert F.
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Sprache:eng
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Zusammenfassung:Gustducin is a transducin-like G protein selectively expressed in taste receptor cells. The α subunit of gustducin (α-gustducin) is critical for transduction of responses to bitter or sweet compounds. We identified a G-protein γ subunit (Gγ13) that colocalized with α-gustducin in taste receptor cells. Of 19 α-gustducin/Gγ13-positive taste receptor cells profiled, all expressed the G protein β3 subunit (Gβ3); ~80% also expressed Gβ1. Gustducin heterotrimers (α-gustducin/Gβ1/Gγ13) were activated by taste cell membranes plus bitter denatonium. Antibodies against Gγ13 blocked the denatonium-induced increase of inositol trisphosphate (IP 3 ) in taste tissue. We conclude that gustducin heterotrimers transduce responses to bitter and sweet compounds via α-gustducin's regulation of phosphodiesterase (PDE) and Gβγ's activation of phospholipase C (PLC).
ISSN:1097-6256
1546-1726
DOI:10.1038/15981