Changes in the content of modified nucleotides in wheat rRNA during greening

Changes in the content of modified nucleotides in wheat rRNA during greening

Changes in the state of modification of 5.8S and 18S rRNAs were examined during the greening of etiolated wheat seedlings. Three types of minor nucleotides, O2'-methyladenosine, O2'-methylguanosine and pseudouridine were found in the 5.8S rRNA of green wheat leaves, none of which was detec...

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Veröffentlicht in:Biologia plantarum 2003-07, Vol.47 (1), p.33-38
Hauptverfasser: Paradi, I, Rudnoy, S, Bratek, Z, Kovacs, G, Racz, I, Lasztity, D. (Eoetvoes Lorand Univ., Budapest (Hungary)), Paldi, E
Format: Artikel
Sprache:eng
Schlagworte:
ADN
AHILAMIENTO
ARN RIBOSOMAL
ARN RIBOSOMIAL
BLÉ
CHROMATOGRAPHIE EN COUCHE MINCE
CROMATOGRAFIA DE CAPA FINA
DNA
ETIOLATION
LIGHT
LUMIÈRE
LUZ
NUCLEOTIDE SEQUENCE
NUCLEOTIDES
NUCLEÓTIDOS
NUCLÉOTIDE
PLANTULAS
PLANTULE
RIBOSOMAL RNA
SECUENCIA NUCLEOTÍDICA
SEEDLINGS
SÉQUENCE NUCLÉOTIDIQUE
THIN LAYER CHROMATOGRAPHY
TRIGO
WHEATS
ÉTIOLEMENT
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Beschreibung
Zusammenfassung:Changes in the state of modification of 5.8S and 18S rRNAs were examined during the greening of etiolated wheat seedlings. Three types of minor nucleotides, O2'-methyladenosine, O2'-methylguanosine and pseudouridine were found in the 5.8S rRNA of green wheat leaves, none of which was detected in etiolated wheat. The minor nucleotides appeared in the 5.8S rRNA only after 48 h irradiation. The sequences of 5.8S rDNA, ITS1, ITS2 and 18S rDNA were also determined and the presence of the hypermodified nucleotide 1-methyl-3-(alpha-amino-alpha-carboxypropyl)-pseudouridine was detected in green wheat 18S rRNA. This minor component was not demonstrable in etiolated wheat 18S rRNA, but appeared after irradiation for 48 h.
ISSN:0006-3134
1573-8264
DOI:10.1023/A:1027320612774